The invention relates to a bimetallic glass mould of copper and stainless steel, belonging to the technical field of moulds. The mould comprises a matrix layer and a mould cavity layer, wherein the matrix layer is made of copper alloy; the mould cavity layer is made of the stainless steel; the copper alloy comprises the following chemical constituents in percentage by weight: 66-68 percent of copper, 2.0-3.0 percent of aluminum and the balance of zinc; and the stainless steel is chromiumnickel austenite stainless steel comprising the following chemical constituents in percentage by weight: atmost 0.08 percent of carbon, at most 1.0 percent of silicon, at most 2.0 percent of manganese, 17-19 percent of chromium, 8-11 percent of nickel, at most 0.03 percent of sulfur and at most 0.035 percent of phosphorus. The invention has the advantages that the glass mould not only can resist instant thermal shock, but also can quickly transfer heat so as to meet the high-speed production requirement of glass containers and have long service life; the method not only can comprehensively embody the effect of the bimetallic glass mould of copper and stainless steel, but also can embody high preparation efficiency, simple process and energy saving.