The invention discloses a breeding and production method of selenium-enriched yeast with high biomass. Through screening and domestication on a culture medium containing inorganic selenium, a strain of Saccharomyces cerevisiae subspecies Saccharomyces boulardii YBN-2 with high biomass and high content of selenium. Under optimal fermentation conditions, each gram of selenium-enriched yeast enriches as high as 2800-3200 mug / g of organic selenium, has viable count reaching 6.5-8.0*10<10> CFU / g. The selenium-enriched yeast provided by the invention has the advanategs of safety, no toxicity, simple production conditions, low cost and high economic value.