The invention discloses a Bacillus subtilis for prevention and control of Fusarium diseases of crops and application thereof. The preservation number of Bacillus subtilis is CGMCC No. 7727. The application means the application of Bacillus subtilis in resisting Fusarium and preventing and controlling scab of wheat, Solanaceae Fusarium wilt and cucurbits Fusarium wilt. Compared with the prior art, the Bacillus subtilis disclosed by the invention has a high antagonistic effect on Fusarium and can be used for preventing and controlling scab of wheat, Solanaceae wilt and cucurbits Fusarium wilt caused by the Fusarium. The prevention efficiency in the field is stabilized at above 60%.