The invention relates to a bacillus subtilis mutant strain capable of producing phospholipase. The invention provides the isolated bacterial strain capable of expressing phosphatidase C, and the mutant strain is preserved in the General Microbiological Center of China Committee of Culture Collection for Microorganisms with a preservation number of CGMCC No. 8500. The provided bacterial strain and phosphatidase C prepared by a preparation method can be used for grease refining, degumming effect is good, and the provided bacterial strain and phosphatidase C can be widely used in the fields of grease refining, additive and medicine.