The invention discloses a nano composite skin disinfection emulsion and a preparation method of the same. The nano composite skin disinfection emulsion is composed of a double-chain quaternary ammonium salt compound, an inorganic nano antibacterial substance, an emulsifier, aloe extract liquid and water, wherein the double-chain quaternary ammonium salt compound is didecyldimethylammonium bromide, didecyldimethylammonium chloride or bis(dodecyldimethyl)ethylene diammonium bromide, and the inorganic nano antibacterial substance is nano zinc oxide, nano silver oxide or nano titanium oxide, and the aloe extract liquid is raw liquid from aloe. The emulsion, when acting on staphylococcus aureus, escherichia coli and candida albicans for 1 min, can reach 100% in killing ratio all. The emulsion is high in inhibiting rates of 6 h, 12 h, 24 h and 48 h on natural bacteria. When acting on hands of a subject for 1 min, the emulsion is higher than 90% in average killing rate on natural bacteria. The emulsion is excellent in stability, can be safely used on human skin and mucosa, and is free of damage and stimulation on environment and human body.