The invention relates to a preparation method and a use of extract for preparation of drugs for promoting myocardial regeneration and belongs to the technical field of Chinese herbal medicines. Water or alcohol extract of labiates Salvia miltiorrhiza Bge dry root or rhizome has effects of specifically inducting and promoting regeneration of myocardial cells in damaged heart so that damaged or necrotic cardiac muscle tissue is replaced and repaired. In a rat myocardial infarction animal model, the extract has effects of promoting cardiac muscle regeneration of the damaged cardiac muscle tissue and repairing cardiac muscle tissue damage. The extract can be processed to form a specific treatment drug for specifically treating myocardial infarction.