The invention belongs to the field of immunobiology, relates to an epitope polypeptide, i.e., a broad-spectrum affinity epitope polypeptide for human enteroviruses, and use thereof and also relates to an antibody capable of being combined with the epitope polypeptide and use thereof. The invention also relates to use of the affinity epitope polypeptide or antibody in the preparation of drugs or methods for treating and/or preventing and/or diagnosing the human enteroviruses and/or assaying the titer of the human enteroviruses and/or assaying the valence of neutralizing antibodies for the human enteroviruses. The affinity epitope polypeptide and the antibody, disclosed by the invention, can be applied to the drugs or methods for treating and/or preventing and/or diagnosing the human enteroviruses and/or assaying the titer of the human enteroviruses and/or assaying the valence of the neutralizing antibodies for the human enteroviruses.