The invention discloses a mask frame assembly for film deposition, a manufacturing method of the mask frame assembly for film deposition, and a manufacturing method of an organic light-emitting display device. The mask frame assembly comprises a frame, a mask and a first fixing component, wherein the frame is provided with an opening and a supporting part. The mask comprises a deposition area at a position which corresponds with the opening. The first fixing component is injected into the mask for penetrating through the mask and furthermore fixing with the frame and the mask for fixing the mask with the frame.