The invention relates to a processing method of fritillaria praewalskii maxim ex batal seeds; the processing method comprises the following steps: A1, screening tawny mature and full seeds, to obtain to-be-treated seeds I; A2, preparing a dilute alcohol acid aqueous solution from deionized water, an organic acid and C2-C4 alcohol; A3, keeping the temperature of the dilute alcohol acid aqueous solution at 20-26 DEG C, then soaking the to-be-treated seeds I in the dilute alcohol acid aqueous solution with the soaking time of 20-30 h, then fishing out, and fully air-drying, to obtain dried seeds; A4, irradiating the dried seeds at the temperature of 10-15 DEG C, in a mixed gas atmosphere and with the irradiation intensity of 3000-3600 lux for 10-14 h, and controlling the relative humidity to be 70-80%, to obtain to-be-treated seeds II; and A5, evenly mixing the to-be-treated seeds II with humus soil according to the mass ratio of 1 to (5-6), and preserving for 15-20 days at the temperature of 8-10 DEG C in dark place to obtain the treated seeds. The invention also relates to a fritillaria praewalskii maxim ex batal culture method. The treatment and culture methods have good application value and promotion potential.