The invention relates to the field of molecular breeding, and particularly relates to a fiber strength related molecular mark from gossypium barbadense and application thereof. The molecular mark is HAU0883125 and TMB1125220, wherein the HAU0883125 is closely linked with the site qFS-C14-1 of cotton fiber strength QTL, and the TMB1125220 is closely linked with the site qFS-C20-1 of cotton fiber strength QTL; and the qFS-C14-1 is located on chromosome C14, and the qFS-C20-1 is located on chromosome C20. The molecular mark can be used for improving the selection efficiency of fiber strength, overcoming the defect in existing breeding technology for fiber quality identification and accelerating the culturing progress of high-quality new varieties.