The invention discloses a preparation for restraining adenovirus infection. The preparation is applied to 1 preparing a product for restraining adenovirus infection and 2 restraining adenovirus infection. The preparation is mainly formed by mixing epigallocatechin gallate, tannin and astragalus polysaccharide. The mass ratio of epigallocatechin gallate to tannin to astragalus polysaccharide is (0.5-1.0):(0.5-1.0):(0.5-1.5). The cytotoxicity of the complex formula preparation is not higher than that of a control sample ribavirin obtaining security permission, and the preparation is safe. Under safe use concentration, type 5 adenovirus infection can be effectively restrained through preventive use of the preparation, and the preparation has commercial value for being further developed into an adenovirus infection inhibitor.