The invention provides a rice pollen high expression promoter OsPoll4 and an application thereof. The invention also provides an expression box containing the promoter, a crop expression vector and a method for driving the expression of a target gene by using the same. Specifically, the inventors find and extract a segment of DNA sequence of 1864bp in Nipponbare and notice that the sequence has ability of driving high expression of the gene in pollen and identify the ability and name the ability OsPoll4. In the invention, the promoter is applied to crop genetic engineering. The promoter provided by the invention can specifically drive the high expression of the exogenous gene in pollen, has a function of analyzing important genes in a pollen development process, and is of important theoretical significance to the rice pollen development and related basic biological study and important practical significance to the transgenic breeding of hybrid rice and paddy.