The invention relates to a 1, 3, 4-thiadiazole liquid crystal compound based on stilbene and a preparation method of 1, 3, 4-thiadiazole liquid crystal compound. The formula of the compound is as shown in formula (I), wherein n=6, 8, 10, 12, 14; R is expressed as F, CN, H, CH3 and CH3O. The compound has the advantages that the compound is good liquid crystal material and is wide in liquid crystal temperature range, high in clearing point, excellent in thermal stability, and the like; the compound has a fluorescence radiation feature, can emit blue fluorescence and is high in fluorescence efficiency; the compound is promising in application prospect in the liquid crystal display field and the photoelectric field.