The invention relates to a seed culture medium for producing vancomycin through fermentation of streptomyces orientalis and a cultivation method. By the adding of composite modified starches, fibroin powder, casein and other substances into the seed culture medium, the seed culture quality and the fermentation unit of vancomycin can be improved favorably, and the fermentation cost can be reduced; furthermore, the influence of the environment on original auxiliary material sources can be reduced to the maximum extent, so that sufficient supply of the original auxiliary material sources can be guaranteed, and efficient production of vancomycin is realized.