The invention relates to a culture medium and a cultivation method for producing lincomycin by fermenting streptomyces lincolnensis. By the use of a seed culture medium formula, a fermentation culture medium formula and a control process which are provided by the invention, the fermentation unit for large-scale production of lincomycin can be increased, and the fermentation cost can be reduced; furthermore, the environmental influence on raw and auxiliary materials can be reduced to the maximum extent; sufficient supply of the materials is guaranteed, and efficient production of lincomycin is realized.