The invention discloses a realtime fluorescent nucleic acid constant temperature amplification detection kit of human cytomegalovirus (HCMV). The detection kit comprises a capture probe, a HCMVT7 primer, an nT7 primer, a HCMV detection probe, M-MLV reverse transcriptase and T7RNA polymerase. The provided method can high specifically, high sensitively, and rapidly carry out nucleic acid amplification detection on samples containing HCMV such as urine, milk, blood, and the like, and little pollution is generated. The provided kit and method can accurately, rapidly, and conveniently carrying out qualitative detection on HCMV; diagnose the virus infection, monitor and predict epidemicity of HCMV, helps the auxiliary diagnosis of virus infection, and observe the drug therapeutic effect.