The invention relates to a biological medical apparatus, in particular to an efficiency card and a preparation method thereof. The efficiency card comprises a substrate, wherein the substrate is coated by a hot sealable film; a 1.0*10<-7> to 8.0*10<-6>mCi 14C source is dispersed into the substrate, and is (NH2)214CO or Na214CO3. The efficiency card has the advantages that the effects of sealing and no leakage are realized, the counting is stably maintained for a long time, a scientific and reasonable quality control plan of a helicobacter pylori (Hp) tester is established, and the product quality is improved.