The invention discloses a molecular marker and a specific primer for identifying wheat grain germination traits and application thereof. The invention provides a specific primer pair. The specific primer pair comprises two primers; amplification products comprise specific DNA molecules; the specific DNA molecules are target sequences of a primer F and a primer R in a wheat genome; the primer F is a single-chain DNA molecule shown as a sequence 3 of a sequence table; the primer R is a single-chain DNA molecule shown as a sequence 4 of the sequence table. The invention also provides a molecular marker related to a wheat germination index; the molecular marker is a DNA fragment prepared by taking the genome DNA of the to-be-detected wheat as a template and amplifying by using the specific primers. The molecular marker and the specific primer have important theoretical significance and economic value for assistant selection of wheat variety with preharvest sprouting resistance through the molecular marker.