The invention discloses a bio-enzyme-wrapped microparticle and a preparation method and application thereof. The bio-enzyme-wrapped microparticle comprises a core, a water-soluble shell, a bio-enzyme-wrapped proliposome and a filling agent filling a space among the core, the water-soluble shell and the bio-enzyme-wrapped proliposome, wherein a bio-enzyme is a composition of glucanase and alkaline protease. According to the bio-enzyme-wrapped microparticle and the preparation method and application thereof, the bio-enzyme can stably exist in various oral care products such as toothpaste and tooth powder through a wrapping technique, and the oral care products have the effects of decomposing dental plaques, removing tooth stain, whitening the teeth and refreshing the oral smell.