The invention relates to Amygdalus ledebouriana Schlecht. TP-M13-SSR core primers and application thereof. The primers have the advantages of being stable in amplification, clear in electrophoretic band and rich in polymorphism. A high-flux low-cost analysis technology system based on the fluorescent sequencing technology in the TP-M13-SSR amplification product detection process is utilized. The invention discloses the Amygdalus ledebouriana Schlecht. TP-M13-SSR primers. The 12 pairs of primers are included. The 12 pairs of selected TP-M13-SSR core primers are evenly distributed in an Amygdalus ledebouriana Schlecht. genome, band patterns are clear and easy to interpret, and the core primers are suitable for detection of common degenerated polyacrylamide gel electrophoresis detection platforms and capillary fluorescence detection platforms; the core primers are high in polymorphism, good in amplification repeatability and completely suitable for research on the aspects of Amygdalus ledebouriana Schlecht. genetic diversity analysis, variety identification, clustering analysis and the like.