The invention belongs to the field of cell culture, and in particular relates to a culture medium for skin keratinocyte and a culture method thereof. The culture medium for the skin keratinocyte provided by the invention comprises a DMEM (Dulbecco Modified Eagle Medium) basic culture medium, an epidermal growth factor, a transforming growth factor, vitamin H, recombinant human insulin, transferrin, hesperidin and resveratrol. By adopting the culture medium for the skin keratinocyte provided by the invention, the cell attachment time of the keratinocyte can be shortened, differentiation of the keratinocyte is prevented, and the pollution of keratinocyte caused by fibroblasts is lowered; meanwhile, by adopting the culture medium for the skin keratinocyte, the proliferation rate of the keratinocyte can be increased, and the vitality and cell characteristics of the keratinocyte in a passage process are maintained; the culture medium is a relatively ideal in-vitro culture medium for the skin keratinocyte.