The invention relates to a non-steroidal anti-inflammatory drug ibuprofen, and concretely relates to an ibuprofen-polypeptide coupling compound and a hydrogel preparation thereof. The ibuprofen-polypeptide coupling compound is connected with ibuprofen and polypeptide through an amide bond. The preparation method of the hydrogel preparation comprises the following steps: dispersing the ibuprofen-polypeptide coupling compound in water, adding a weak base aqueous solution, heating the material, and completely dissolving the material, and cooling to obtain an ibuprofen-polypeptide coupling compound hydrogel system. The hydrogel preparation prepared by the ibuprofen-polypeptide coupling compound has the advantages of simple preparation, fast gelling, accurate quantitative controllable drug loading capacity, and controllable drug release.