The invention relates to a marine bacterial gene LfliZ and an application thereof. The nucleotide sequence of the marine bacterial gene LfliZ is shown as SEQ ID NO.1. The invention further relates to recombinant protein LfliZ expressed by the gene LfliZ and an application of the marine bacterial gene LfliZ in preparation of 2',3'-circular nucleoside monophosphate. The protein LfliZ coded by the cloned gene LfliZ can be combined with four kinds of 2',3'-circular nucleoside monophosphate in escherichia coli cells. The four kinds of 2',3'-circular nucleoside monophosphate can be extracted directly and simultaneously from escherichia coli by purifying the recombinant protein LfliZ. Calculation proves that about 2.5 mg of the four kinds of pure 2',3'-circular nucleoside monophosphate can be extracted from each liter of a culture solution, and better application potential is provided.