The invention discloses a functional regeneration membrane capable of guiding muscle tissue regeneration as well as a preparation method and applications of the functional regeneration membrane. The regeneration membrane is prepared through electrostatic spinning by taking polycaprolactone as the main base material. Preferably, a compact poly-noradrenaline layer is formed on the membrane through polymerization reaction, and then protein capable of promoting cell growth is combined for use. The membrane material provided by the invention is characterized by being a multi-layer porous membrane material composed of fibers with the diameter being 1-20 [mu] m. The regeneration membrane is simple in preparation technology, can effectively promote the regeneration and repair of the muscle tissue, has good biocompatibility and excellent mechanical property, and can be widely applied to the medical fields of the guidance on skin damage repairing, the guidance on muscle damage repairing, drug slow-release membranes, and the like.