The invention belongs to the field of biochemistry, and relates to an embryo taking buffer liquid and an application thereof; the embryo taking buffer liquid is prepared by the following steps: carrying out water bath of the embryo taking buffer liquid for standby application, and adding a PBS buffer liquid into a culture dish; taking a refrigerated uterine tissue to remove to a constant temperature stage; on the constant temperature stage, placing another culture dish and the uterine tissue, cutting off uterine mesentery, cutting cornua uteri open at a distal fallopian tube, nipping the distal cornua uteri tightly, cutting proximal cornua uteri open, dividing the uterus into two parts, at a cut-open cornua uteri proximal end, injecting the embryo taking buffer liquid, swashing the cornua uteri, starting from a cornua uteri distal end, extruding cornua uteri contents comprising the embryo taking buffer liquid to the cornua uteri proximal end into the culture dish; and removing the culture dish to under a stereoscope, searching embryos, separating the embryos one by one to put in a culture dish having addition of a PBS buffer liquid, rinsing, numbering and recording. The embryos can be efficiently harvested, the vitality of the harvested embryos can be guaranteed, and the harvested embryos can be used for study of cell culture and auxology and thremmatology.