The invention discloses a recombinant vector and method for expressing an F protein of respiratory syncytial virus. The recombinant vector comprises a framework vector and an F protein expression frame of the respiratory syncytial virus, wherein the F protein expression frame of the respiratory syncytial virus is inserted into the framework vector and located at the downstream of a vector promoter and comprises an F2 protein gene sequence and an F1 protein gene sequence, and the F2 protein gene sequence and the F1 protein gene sequence are connected through a 2A sequence. The recombinant vector provided by the invention can simultaneously express multiple genes, and the expressed protein can be efficiently and automatically sheared into F1 and F2 so as to form an F1-F2 homotrimer. The purified F protein can be efficiently prepared by virtue of the method and can be used as an antigen for inducing the high-level antibody immunoreaction.