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CN106119388A 一种鉴别百合品种黄天霸的PCR引物对、试剂盒和方法 失效 - 权利终止

一种鉴别百合品种黄天霸的PCR引物对、试剂盒和方法
Abstract:
本发明属于基因工程领域，具体涉及一种鉴别百合品种黄天霸的PCR引物对、试剂盒和方法。该PCR引物对，其中的一条引物具有序列表中的SEQ ID NO:1的核苷酸序列，另一条引物具有序列表中的SEQ ID NO:2的核苷酸序列；或其中的一条引物具有序列表中的SEQ ID NO:3的核苷酸序列，另一条引物具有序列表中的SEQ ID NO:4的核苷酸序列。该试剂盒包括该PCR引物对。该方法包括：PCR扩增反应和检测步骤。本发明可以从分子水平上鉴别出该待测产物是否是百合品种黄天霸，相比从外观上鉴别更加准确。
Abstract(English):

The invention belongs to the field of genetic engineering, in particular to a PCR (Polymerase Chain Reaction) primer pair, a kit and a method for identifying lily variety mantissa. One primer in the primer pair has a nucleotide sequence with SEQ ID NO:1 in a sequence table, and the other primer has a nucleotide sequence with SEQ ID NO:2 in the sequence table; or one primer has a nucleotide sequence with SEQ ID NO:3 in the sequence table, and the other primer has a nucleotide sequence with SEQ ID NO:4 in the sequence table. The kit comprises the PCR primer pair. The method comprises PCR amplified reaction and a detecting step. According to the PCR primer pair, the kit and the method disclosed by the invention, whether a product to be detected is the lily variety mantissa or not can be identified from a molecular level; compared with a mode of identifying from the appearance, the mode disclosed by the invention is more accurate.

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