The invention discloses a venous transfusion permeation prevention device for operating room nursing. The device comprises a base, wherein a telescopic rod is arranged on the upper surface of the base; a tightness switch is arranged on the side surface of the telescopic rod; a transfusion workbench is arranged above the telescopic rod; the lower surface of the transfusion workbench is fixedly connected with the upper end face of the telescopic rod; a supporting rod is arranged on the upper surface of the transfusion workbench; a hook is fixedly connected to the top end of the supporting rod; a monitor case is arranged on one side of the supporting rod; a sleeve is arranged on one side of the monitor case; a piston rod is arranged inside the sleeve; one end of the piston rod is matched with the sleeve, and the other end of the piston rod is fixedly connected with one end face of a connecting block; the other end face of the connecting block is fixedly connected with one end of a spring; and the side face of the connecting block is fixedly connected with one end of a second transverse rod. The whole venous transfusion permeation prevention device is simple in structure and convenient to use, and can be used for effectively preventing occurrence of permeation of venous transfusion, so that the nursing safety can be greatly improved.