The invention discloses gas reference material cylinder cleaning equipment and belongs to the technical field of gas reference materials. The gas reference material cylinder cleaning equipment comprises a treatment cabin, a vacuum pump, an absorber, a heating belt and the like, wherein the a cylinder holder, a valve cavity and a lifting mechanism are arranged in the treatment cabin; the cylinder holder is used for carrying at least one gas reference material cylinder; when the gas reference material cylinder is cleaned, the valve cavity and the gas reference material cylinder are controlled by the lifting mechanism to move oppositely, so that a valve of the gas reference material cylinder can be positioned in the valve cavity; and quick couplings of which one is used for being connected with the valve of the gas reference material cylinder are arranged in the valve cavity. By adoption of the gas reference material cylinder cleaning equipment, the valve can be protected from deformation caused by too high temperature and the seal performance of the valve can be guaranteed when the cylinder body of the gas reference material cylinder can reach high temperature.