The invention relates to a preparation method of 1-pyrenol. The preparation method comprises the following steps of warming an alkyl sodium alkoxide solution for reflux, adding 1-bromine pyrene, performing a reaction for 10-15 hours, recovering a solvent, lowering the temperature, and performing centrifugal drying so as to obtain 1-alkoxyl pyrene; dissolving the 1-alkoxyl pyrene in the solvent, enabling the dissolved 1-alkoxyl pyrene and an amino acid compound containing tail-end sulfydryl to be subjected to a reaction under the condition of inorganic alkali and the temperature rising to 120-170 DEG C for 2-3 hours, recovering the solvent, performing purifying, and performing drying so as to obtain the 1-pyrenol. According to the 1-pyrenol prepared by the method disclosed by the invention, the content is 99%, and the yield is 88% or above.