The invention relates to broad spectrum pathogenic bacterium resisting bacillus subtilis and application thereof, and belongs to the technical field of microorganisms. The conservation serial number of the disclosed bacillus subtilis is CGMCC 11164. The bacillus subtilis has the capacity for inhibiting pathogenic bacteria mainly including escherichia coli, staphylococcus aureus, salmonella typhimurium, Shigella, alpha-hemolytic streptococcus and beta-hemolytic streptococcus in a broad spectrum mode, has high capacity for producing protease, amylase and cellulase, and has excellent application prospects in feed and microbial agents.