The present invention provides a differential sensing circuit (200) with a dynamic voltage reference for a single-ended bit line memory (202) is disclosed. The exemplary differential sensing circuit (200) comprises: a dynamic voltage reference generating unit (210) and a differential sensing amplifying unit (220). The dynamic voltage reference generating unit (210) is coupled to an input voltage, and utilized for receiving a setting signal to generate the dynamic voltage reference. The differential sensing amplifying unit (220) is coupled to the single-ended bit line memory (202) and the dynamic voltage reference generating unit (210), and utilized for receiving at least an input signal from the single-ended bit line memory (202) and the dynamic voltage reference from the dynamic voltage reference generating unit (210), so as to generate at least an output signal accordingly.