The invention discloses a tebuconazole molecularly imprinted membrane electrode based on nano gold sensibilization, and preparation, application and test methods of an electrochemical sensor of the tebuconazole molecularly imprinted membrane electrode. Gold nanoparticles are settled on the surface of the electrode first to serve as a substrate, and an electropolymerization membrane with a controllable membrane thickness is prepared on the surface of the electrode by adopting an in-situ electric polymerization method, so that the tebuconazole molecularly imprinted membrane electrode with a recognition site and a hole is obtained; the electrode is a gold electrode or a glassy carbon electrode. The method comprises the following steps: (1) electrode pretreatment; (2) settlement of the gold nanoparticles; (3) preparation of the molecularly imprinted membrane; (4) removal of a molecular template. The electrochemical sensor is applied to selective measurement of tebuconazole in a fruit or vegetable sample. A test comprises the following steps: (a) extracting a target molecule, and (b) performing electrochemical test. According to the tebuconazole molecularly imprinted membrane electrode based on nano gold sensibilization, the gold nano particles are settled on the surface of the gold electrode or the glassy carbon electrode to serve as the substrate, and the specific molecularly imprinted membrane is formed, so that the detection specificity, the sensitivity and the response of measuring the tebuconazole in the fruit or vegetable sample are greatly improved.