The invention provides an identification method and identification device of a business flow. The identification method of the business flow comprises the following steps: analyzing a business flow data packet at a terminal side to obtain the first IP quintuple information; analyzing a network process file in a terminal operation system to obtain the corresponding relation between the IP quintuple information and an application; determining the application corresponding to the first IP quintuple information according to the corresponding relation between the IP quintuple information and the application so as to identify the application corresponding to the business flow. Through the scheme provided by the invention, the business flow can be fast and accurately identified, the corresponding relation between the business flow and the application is obtained, and the scheme is suitable for various different applications.