The invention relates to a preparation method of high sporopollen of beauveria bassiana. The preparation method comprises the steps of mixing hypha fermentation liquid of beauveria bassiana with a nutrient solution subjected to high-temperature steam sterilization and cooled to the room temperature in a proportion of 1 to (1-3) to obtain a culture solution, putting a cloth block or a sponge block soaked with the culture solution to a stackable fermentation container, putting the fermentation container in a fermentation chamber with the temperature of 25-28 DEG C, and fermenting for 5-6 days, wherein through holes are formed in the bottom of the fermentation container, and the nutrient solution is prepared from the following raw materials in parts by weight: 1000 parts of water, 20 parts of corn flour, 20 parts of rice flour and 40 parts of white granulated sugar. The preparation method has the advantages that the production efficiency is high, the fermentation period is short, and conidia and a matrix can be easily separated; and the spore content of the prepared high sporopollen of beauveria bassiana is 8.0*10<10>spore/g-9.5*10<10>spore/g.