The invention relates to a novel high-strength high-hydrophilia oxidized graphene-P34HB nanofiber scaffold and a preparing method and application thereof. The method includes the steps that (1) P34HB is modified with oxidized graphene, and an oxidized graphene-P34HB modification electrospinning solution is obtained; (2) the oxidized graphene-P34HB modification electrospinning solution is subjected to electrostatic spinning with the electrospinning technology, and the modified nanofiber membrane scaffold is obtained. According to the novel high-strength high-hydrophilia oxidized graphene-P34HB nanofiber scaffold and the preparing method and application, the P34HB is modified with the oxidized graphene for the first time, the modified biological scaffold is prepared with the electrospinning technology, the mechanical performance of the scaffold is greatly improved, and meanwhile the hydrophilia of the scaffold is improved; fibers of the scaffold are continuous, collapse and adhesion are avoided, and the porosity up to 91.4%, the high specific surface area and excellent hole communication performance are achieved; the scaffold has better biosecurity, and meanwhile has certain osteogenic induction capacity.