The invention provides a semi-automatic biochemical immunoassay analyzer in reproductive medicine. The semi-automatic biochemical immunoassay analyzer comprises a constant temperature system with air circulation incubation, and a cold light source optical measuring system, wherein the constant temperature system with air circulation incubation is connected with the sold light source optical measuring system through an optical fiber. The semi-automatic biochemical immunoassay analyzer has the beneficial effects that five or more than five LED cold light sources are used as measuring light sources, the requirement of seminal fluid biochemical immunoassay project measurement for measuring wavelength is met, each cold light source is long in service life, the calorific value in the using process is low, and the integral maintenance cost of the instrument is greatly reduced; a constant temperature incubating system is integrated in the measuring system, and the uniformity of incubating environment temperature is improved, so that the accuracy and reliability of a test result are improved, and a beneficial clinical effect is reached; a great deal of cost is reduced, and the quantity of laboratorial equipment is reduced, so that the area of occupied space is reduced; meanwhile the workload of inspection personnel is also greatly alleviated, and the work efficiency is improved.