The invention belongs to the technical field of forensic medicine, and particularly relates to a forensic medicine II sequence testing kit based on 74 gama chromosome SNP genetic markers. The technical problem to be solved is to classify detection materials of Chinese group source at the position of a gama chromosome evolution tree by using the gama chromosome SNP genetic markers. The technical scheme of the invention is forensic medicine II sequence testing kit based on 74 gama chromosome SNP genetic markers, which includes a mixture of 72 pairs of primers for recombination and amplification, thus the detection of 74 pieces of SNP at the same time becomes true. The kit applies single pipe internal recombination and amplification and II sequence testing technology, and thus the genetic typing of 74 pieces of gama chromosome SNP genetic markers of multiple biological detection materials can be obtained in one time; besides, male samples of Chinese group source are correctly affiliated to the branch of the recognized gama chromosome evolution tree.