The invention relates to a preservation method for fruits and vegetables, in particular to a biological preservation method for fruits and vegetables. The biological preservation method includes the steps: (1) harvesting, classifying and screening the fruits and the vegetables; (2) treating microorganism source volatile components; (3) treating plant source volatile essential oil; (4) treating animal source lysozyme; (5) packaging and warehousing. The preservation method takes perishable fresh agricultural products such as strawberries, potatoes, kiwi fruits and garlic as objects and takes storage problems such as gray mold, dry rot and sprouting as prevention and control targets, biological preservation technique level is achieved by the aid of a product anticorrosion preservation way and a technical anticorrosion preservation way, and edible safety of the fresh agricultural products is improved.