The invention discloses a pilot scale production method of white zein and belongs to the technical field of deep processing of corn gluten meal. The pilot scale production method of the white zein comprises the steps of firstly removing pigments and fats in the corn gluten meal with a sub-critical fluid extraction technology, and desolventizing after the sub-critical fluid extraction to obtain the discolored and defatted corn gluten meal; then extracting the discolored and defatted corn gluten meal with an ultrasonic dynamic countercurrent extraction technology to obtain a zein extracting solution, obtaining the zein wet powder by vacuum low-temperature concentration after filtering, and finally obtaining the white zein product by vacuum freezing and drying. The manufactured zein is a white solid, so that the application of the zein is no longer limited by a specific color, and will be widely applied to the industries of food and medicine. The additional value of the corn gluten meal is significantly increased, and the comprehensive development and utilization of the corn gluten meal are benefited. The pilot scale production method of the white zein has the advantages of being simple, efficient, environment friendly and low in cost and the like, and is suitable for mass production.