The invention discloses an H11 fixed-point knock-in heterozygous mice model of a conditional overexpression Spp1 gene and a construction method of the H11 fixed-point knock-in heterozygous mice model of the conditional overexpression Spp1 gene. The construction method includes obtaining an inducible Spp1 overexpression transgenic vector; obtaining transgenic F0-generation mice; enabling the transgenic F0-generation mice to mate with wild-type mice for passage and line establishment so as to obtain F1-generation mice; selecting the F1-generation mice high in expression in terms of bone marrows, spleens, lymph nodes and thymus glands as a subsequent laboratory strain so as to obtain transgenosis tool mice; enabling the transgenosis tool mice to mate with Cre transgenic mice so as to obtain the H11 fixed-point knock-in heterozygous mice model. The H11 fixed-point knock-in heterozygous mice model, a transgenosis mice model, is an inducible leukemia model characterized in that Spp1 isn't in expression and causes no leukemia in the absence of an inducer and is in expression and causes leukemia in the presence of the inducer, thereby being higher in controllability than a conventional transgenosis mice model and capable of adjusting the quantity of species needing to be maintained according to experiment needs.