The invention discloses a chromatographic column sealing assembly. The chromatographic column sealing assembly comprises a tightening ring, a supporting ring and a sealing ring, which are arranged coaxially in sequence from top to bottom, wherein the tightening ring and the supporting ring are elastically connected; the supporting ring and the sealing ring are made of ferromagnetic materials; and an annular permanent magnet is coaxially arranged between the supporting ring and the sealing ring. The chromatographic column sealing assembly disclosed by the invention has the advantages of simplicity in detachment, washing and assembling and good sealing effect, and has the beneficial effect of greatly reducing the probability that a chromatographic column is suffered from secondary pollution in a butt joint injection process.