The invention relates to a novel lentivirus packaging method. The lentivirus packaging method comprises the following steps: (1) inoculating 2*10<6>293T cells; (2) cultivating transfected mixture for 12-15 hours; (3) mixing each transfection centrifugal tube, wherein virus vectors are 10 microg of small-fragment RNA plasmid, 40 microg of package plasmid and 20 microg of enveloped protein plasmid, making up to 100 microL with 100mM sodium chloride; (4) preparing mixed solution of Biomiga Genetran III and sodium chloride; (5) adding 80 microL Biomiga Genetran III and sodium chloride mixed solution into the transfection centrifugal tube, wherein the total volume is 180 microL; (6) standing for 20-30 minutes at room temperature; (7) sucking culture medium from cells, then adding 8ml of fresh culture medium, adding 50 microL of sec-butyl babian within half an hour and mixing evenly; (8) culturing for 48-60 hours; and (9) collecting viruses after 72 hours.