The invention relates to a serratia marcescens fermentation liquor culture medium promoting growth and development of hypsizygus marmoreus. The culture medium is prepared to obtain serratia marcescens fermentation liquor, the serratia marcescens fermentation liquor is added into an ordinary hypsizygus marmoreus culture medium, and the hypsizygus marmoreus is cultivated. The method can promote growth of hypsizygus marmoreus hyphae and secretion of manganese peroxidase, cellulase and xylanase. Preparation of the serratia marcescens fermentation liquor and the optimum volume ratio of addition are determined, and measurement of the growth of the hypsizygus marmoreus hyphae and secretion of the manganese peroxidase, the cellulase and the xylanase proves that the culture medium promotes growth and development of the hypsizygus marmoreus. The phenomenon that serratia marcescens is utilized for promoting growth and development of the hypsizygus marmoreus has not been reported.