The invention discloses an application of a thiamphenicol molecularly imprinted electrochemical sensor. A molecularly imprinted membrane-modified L-shaped glassy carbon electrode is immersed into a thiamphenicol-containing sample, identified, and then washed with water to remove non-specifically adsorbed molecules; a three-electrode system is formed by taking the recognized molecularly imprinted membrane modified L-shaped glassy carbon electrode as a working electrode, a saturated calomel electrode as a reference electrode and a platinum wire electrode as a counter electrode, and is soaked into a phosphate buffer solution which contains 0.1 mol L<-1> ascorbic acid and is in the pH value of 7.0 by taking a 2cm cuvette as a photoelectric detection pool; 405nm laser is irradiated on the surface of the working electrode, AA generates photocurrent, and a working curve is obtained according to the fact that the variation of the photocurrent is in direct proportion to the concentration of thiamphenicol. The sensor can rapidly and accurately detect thiamphenicol in meat samples and feed samples.