The invention relates to a rotating disc type laser scanning reading device. The device comprises: a cassette; a cassette heater driven by a motor, wherein a magnet is embedded in the heater, and the control mechanism of the motor is characterized in that a screw right end portion stretching out of a cassette body in the cassette and a press plate shaft right end portion stretching out of the cassette body are connected with the motor, and a cassette sampling needle is controlled by the motor to move transversely and vertically; an integrated circuit board; a bar code reader; and a scanner comprising a high speed rotating platform, a laser emitter and a photomultiplier, wherein a chip at the bottom of the cassette moves on a rotating disc at a high speed, and the laser emitter and the photomultiplier are oppositely arranged. All reagents needed by nucleic acid extraction, amplification and detection are preplaced in the cassette to avoid pollution; and a high-concentration PCR product is sealed in the cassette, so a pollution source is effectively controlled. The device integrates nucleic acid extraction, amplification, detection and scanning, and realizes automation and sealing.