The invention relates to the technical field of medical guiding puncture, in particular to a sheath for atrial septal puncture. The part stretching into human body tissue is provided with a working end, the working end is provided with electric conductors, wires are connected to the electric conductors, the other ends of the wires extend to the exterior of the human body tissue along the sheath and are connected with a signal receiving device, and the sheath is arranged in an electric field. Accordingly, the sheath can be accurately positioned, the puncture success rate is increased, and the patient pain is reduced.