The invention provides a method for reducing Maillard reaction during a culture medium sterilization process. The method comprises the following steps: adding binary acid and monohydric alcohol into a prepared culture medium, carrying out stirring, carrying out sterilizing, carrying out cooling till the temperature is 50-100 DEG C, introducing sterile gas and enabling the sterile gas to pass through the culture medium, stopping stirring, and carrying out further cooling, wherein after layering of the system, an organic phase and a solid phase are separated out, and the culture medium is used for fermentation. With the adoption of the method provided by the invention, the Maillard reaction flux in the system can be effectively reduced, meanwhile, an intermediate product and a final product of the Maillard reaction are separated from the system through the extraction principle, compared with the discontinuous sterilization process, the one-step sterilization process provided by the invention has the advantages that the construction cost of the fermentation device is reduced by one half, the energy consumed during the sterilization process is reduced by one half, and thus the construction cost and operation cost of the device are obviously saved.