The invention relates to pseudomonas aeruginosa conjugated with dioxin degradation plasmid as well as a culture method and an application of the pseudomonas aeruginosa. The pseudomonas aeruginosa SJCJ-1 conjugated with the dioxin degradation plasmid is preserved in China General Microbiological Culture Collection Center on August 8, 2016 with preservation number of CGMCC No.12828. The invention also relates to the culture method and the application of the strain. The pseudomonas aeruginosa strain, which is capable of degrading such compounds as dibenzofuran, chlorinated didenzofuran and the like, can be obtained by combining gram-positive bacterium, namely rhodococcus rhodococcus p52, and gram-negative bacterium, namely pseudomonas aeruginosa; the strain is applicable to a sewage treatment or soil bioremediation process and the strain can grow rapidly, and the degradation plasmid in the strain can still keep 50% cells after continuous passage for 30 generations under a non-selective-pressure condition, so that a strong stability is guaranteed.