The invention discloses a lipase immobilization method. The lipase immobilization method comprises the steps that lipase, a protein linking peptide and a chitosan binding domain amino acid sequence are sequentially connected to form a lipase fused protein, chitosan is used as a carrier to fix the lipase fused protein, and fixed lipase is obtained. The fixed lipase is subjected to recycling of fifth times, the conversion rate of cetyl palmitate catalyzed by the fixed lipase fused protein can be still kept 90%. The method can achieve large scale production, the lipase production can be improved, and the production cost can be reduced.